Diffraction phase and fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Diffraction phase and fluorescence microscopy.
We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-l...
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We present spectroscopic diffraction phase microscopy (sDPM) as a method capable of measuring quantitative phase images at multiple wavelengths. sDPM uses a spatial light modulator at the Fourier plane of a lens to select desired wavelengths from the white light illumination of a grating. The quantitative phase information at different wavelengths allows us to decouple the refractive index and ...
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Fluorescence imaging with conventional microscopy has experienced numerous advances in almost every limiting factor from sensitivity to speed. But improved resolution beyond the fundamental limitation of light diffraction has been elusive until recent years. Now, techniques are available that surpass this barrier and improve resolution up to 10 times over that of conventional microscopy. This c...
متن کاملWhite Light Diffraction Phase Microscopy
Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy due to measurement of phase information quantitatively. In QPI techniques, there is no need of exogenous contrast agents to stain or tag the specimen. White light diffraction ...
متن کاملDiffraction phase microscopy with white light.
We present white light diffraction phase microscopy (wDPM) as a quantitative phase imaging method that combines the single shot measurement benefit associated with off-axis methods, high temporal phase stability associated with common path geometries, and high spatial phase sensitivity due to the white light illumination. We propose a spatiotemporal filtering method that pushes the limit of the...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2006
ISSN: 1094-4087
DOI: 10.1364/oe.14.008263